A synthetic RNA editing factor edits its target site in chloroplasts and bacteria

Abstract Members of the pentatricopeptide repeat (PPR) protein family act as specificity factors in C-to-U RNA editing. The expansion PPR superfamily plants provides sequence variation required for design consensus-based RNA-binding proteins. We used this approach to a synthetic editing factor target one sites Arabidopsis chloroplast transcriptome recognised by natural CHLOROPLAST BIOGENESIS 19 (CLB19). show that our specifically recognises vitro binding assays. designed is equally specific rpoA site when expressed chloroplasts and bacterium E. coli. This study serves successful pilot into application programmable based on plant